[Download] "Enrichment of Tetranucleotide Microsatellite Loci from Invertebrate Species." by Journal of Shellfish Research ~ eBook PDF Kindle ePub Free

📘 Read Now     📥 Download

eBook details

• Title: Enrichment of Tetranucleotide Microsatellite Loci from Invertebrate Species.
• Author : Journal of Shellfish Research
• Release Date : January 01, 2004
• Genre: Life Sciences,Books,Science & Nature,
• Pages : * pages
• Size : 202 KB

Description

ABSTRACT An experimental procedure using biotin labelled probes and streptavidin bound magnetic beads was developed to produce microsatellite-enriched libraries in two bivalve species, geoduck and Japanese scallop, and one crustacean, Dungeness crab. Microsatellite libraries were enriched tar GAT[A.sub.4] and GAC[A.sub.4] by 75 84%. The magnetic bead protocol produced genomic inserts ranging in size from 347-449 up. with an average 5' and 3' flanking of 89-130 bp. Eight polymorphic loci were isolated from both geoduck dam and Dungeness crab. The heterozygosities ranged from 0.57-0.92 for Dungeness crab and 0.91-0.97 for geoduck. These novel polymorphic microsatellite loci will be useful in studying recruitment dynamics and genetic structure. KEY WORDS: microsatellites, enrichment, magnetic beads, geoduck, crab, scallop

Ebook Download "Enrichment of Tetranucleotide Microsatellite Loci from Invertebrate Species." PDF ePub Kindle